Peptides are increasingly gaining traction in various research fields. To achieve reliable research outcomes, it is beneficial to understand basic peptide storage and reconstitution. These are some elemental tips for optimal peptide storage and handling.
FOR IN VITRO RESEARCH PURPOSES ONLY.
NOT FOR MEDICAL, HUMAN OR VETERINARY USE.
PEPTIDE STORAGE
Proper storage is vital for maintaining stability and integrity of peptides. Peptides in their lyophilised (freeze-dried) form are stable and safe for transport. We recommend refrigeration as soon as possible upon arrival to ensure proper storage and extend their longevity. This stands for basic, acidic, neutral, hydrophobic and hydrophilic peptides
Lyophilised peptides stored at -20°C in a freezer will remain stable for up to 48 months, making this ideal for long-term storage.
Once reconstituted, peptides will remain stable for 2-8 weeks when refrigerated at 2-8°C.
Note: Peptides naturally degrade, gradually, over time. Degradation doesn’t mean reduced integrity initially. However, over time, peptides can eventually fragment and lose structural integrity.
Peptide Reconstitution
Peptides are supplied in lyophilised powder form, requiring reconstitution. For best results, allow both the peptide vial and the mixing solution (e.g. bacteriostatic water) to reach room temperature before mixing. This helps avoid issues like cloudiness that may occur when combining cold constituents.
EQUALISING VIAL PRESSURE
When reconstituting, equalising pressure in the vial is important. Dependent on drying process, some vials may have an internal vacuum or positive pressure. To counteract this, insert a sterile needle, half-full with air, into the vial’s rubber stopper. The vial will either draw in or release air, stabilising the pressure. This lowers risk of possible solution loss.
MIXING
Bacteriostatic water is most commonly used for peptide reconstitution.
For hydrophobic peptides, a 50% aqueous acetic acid solution can be advantageous.
For mixing technique, it is advisable to gently swirl the vial until the peptide powder is fully dissolved. Avoid vigorous shaking, as this can damage the peptide structure.
Avoid Excessive Solution Volumes
Using too much solution can compromise peptide structure. Using 1-2.5 ml of solution for reconstitution is usually sufficient, unless specific studies confirm otherwise. Excessive dilution may compromise peptide integrity, potentially reducing efficacy.
LIMITATION OF LIABILITY
Under no circumstances shall this company be held liable for any loss, damage, injury, or legal action arising from the misuse, improper handling, or unauthorised consumption of our products. Any deviation from the strictly intended research use is prohibited and performed entirely at the user’s own risk.
FOR IN VITRO RESEARCH PURPOSES ONLY.
NOT FOR MEDICAL, HUMAN OR VETERINARY USE.